Microcallus formation from maize protoplasts prepared from embryogenic callus
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چکیده
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AtWuschel Promotes Formation of the Embryogenic Callus in Gossypium hirsutum
Upland cotton (Gossypium hirsutum) is one of the most recalcitrant species for in vitro plant regeneration through somatic embryogenesis. Callus from only a few cultivars can produce embryogenic callus (EC), but the mechanism is not well elucidated. Here we screened a cultivar, CRI24, with high efficiency of EC produce. The expression of genes relevant to EC production was analyzed between the ...
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In North Carolina, approximately 8% of the cucumber (Cucumis sativus) yield is lost to root-knot nematodes (Meloidogyne spp.) (Main and Gurtz, 1989). No resistance was found in cucumber after screening of 900 cultigens for resistance to Meloidogyne incognita race 3 (Walters, 1991). African horned cucumber (Cucumis metuliferus) has resistance to M. incognita (Fassuliotis, 1967; Walters, 1991), a...
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Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration of the core, particularily the nuclear mass. Since remnant spore coat covered s...
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Protoplasts isolated from root cap cells of maize were shown to secrete fucose-rich polysaccharides and were used in a patch–clamp study to monitor changes in whole-cell capacitance. Ca 2 1 was required for exocytosis, which was measured as an increase in cell capacitance during intracellular dialysis with Ca 2 1 buffers via the patch pipette. Exocytosis was stimulated significantly by small in...
متن کاملThe Phaseolus vulgaris PvTRX1h gene regulates plant hormone biosynthesis in embryogenic callus from common bean
Common bean is the most important grain legume in the human diet. Bean improvement efforts have been focused on classical breeding techniques because bean is recalcitrant to both somatic embryogenesis and in vitro regeneration. This study was undertaken to better understand the process of somatic embryogenesis in the common bean. We focused on the mechanisms by which somatic embryogenesis in pl...
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ژورنال
عنوان ژورنال: Planta
سال: 1986
ISSN: 0032-0935,1432-2048
DOI: 10.1007/bf00392367